In this study, we present new metal complexes of Cr(II), Co(II), and Ni(II) synthesized using novel ligands produced through azo coupling between luminol and sulfamethoxazole. UV-Vis, FT-IR, ¹H NMR, and ¹³C NMR spectroscopy were used to characterize the produced ligands and their complexes. UV-Vis results indicate that the maximum difference in absorbance wavelengths (λ max) was observed for the azo dye ligand and its respective metal complex, indicating successful coordination. Calibration curves constructed at the wavelengths specified for each complex are reported to exhibit excellent linearity within the range of 1.0-20.0 mg mL-1 (R²> 0.998). The limits of detection LOD and quantification LOQ were determined to be 0.18 and 0.55 μg mL-1, respectively. The method met the conditions for precision, as expressed by the relative standard deviation (RSD%), and was thus shown to have high reproducibility, with an RSD% below 2.5% (n = 5). Recovery studies were performed using known standards to spike analytes with recoveries ranging from 97.2% to 102.1%, indicating the method is accurate. The complexes were stable under varying pH, temperature, and time conditions. Job's and molar ratio methods confirmed 1:2 metal-to-ligand stoichiometry. Furthermore, the synthesized complexes were also found to exhibit considerable antibacterial activity against both Gram-positive and Gram-negative bacteria, in addition to the above analytical reliability in the new luminol-based azo complexes. New metal complexes (CrII, CoII, and NiII) were constructed using novel ligands synthesized from the azo coupling reaction between luminol and sulfamethoxazole. Calibration curves for the λmax were utilized to study the adherence of the complexes to the Beer-Lambert law.
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Ali**,B. Khalil (2025). Evaluation and analysis of new complexes of luminol as a chemiluminescent material for biological samples by spectroscopy methods. Analytical Methods in Environmental Chemistry Journal, 8(3), 31-46. doi: 10.24200/amecj.v8.i03.1030
MLA
Ali**,B. Khalil. "Evaluation and analysis of new complexes of luminol as a chemiluminescent material for biological samples by spectroscopy methods", Analytical Methods in Environmental Chemistry Journal, 8, 3, 2025, 31-46. doi: 10.24200/amecj.v8.i03.1030
HARVARD
Ali** B. Khalil (2025). 'Evaluation and analysis of new complexes of luminol as a chemiluminescent material for biological samples by spectroscopy methods', Analytical Methods in Environmental Chemistry Journal, 8(3), pp. 31-46. doi: 10.24200/amecj.v8.i03.1030
CHICAGO
B. Khalil Ali**, "Evaluation and analysis of new complexes of luminol as a chemiluminescent material for biological samples by spectroscopy methods," Analytical Methods in Environmental Chemistry Journal, 8 3 (2025): 31-46, doi: 10.24200/amecj.v8.i03.1030
VANCOUVER
Ali** B. Khalil Evaluation and analysis of new complexes of luminol as a chemiluminescent material for biological samples by spectroscopy methods. Anal. Method Environ. Chem. J., 2025; 8(3): 31-46. doi: 10.24200/amecj.v8.i03.1030
