Analytical Methods in Environmental Chemistry Journal
AMECJ

Analysis and the determination of prednisolone in pharmaceutical products by a high-performance liquid chromatography method

Document Type : Original Article

Authors

Fadhil Ibrahim Aljabari** 1
Saed A. Alani 2
Zaid A. Alndawi 3

1 Department of chemistry, Baghdad University College of science for women, Baghdad, Iraq

2 Department of Chemistry, Baghdad University College of Ibn al-Haithem, Baghdad, Iraq

3 College of Health and Medical Techniques, Almustaqbal University, Hilla, Iraq

https://doi.org/10.24200/amecj.v8.i03.1083
Abstract
Prednisolone is essential because it is a potent corticosteroid medication with anti-inflammatory and immunosuppressive effects, widely used in both human and veterinary medicine. Therefore, accurate measurement is crucial for determining the correct human dose. A fast and high-performance liquid chromatography (HPLC) method was developed to determine prednisolone in both its pure form and pharmaceutical products. It consists of a 4.6 mm x 250 mm column (C18 packed with 5µm particles), with the mobile phase being (CH3OH: H2O) in a ratio of 58:42 v/v and a flow rate of 1 mL per minute, using an automatic injector (20 µL). The detection was performed at 254 nm with a UV detector. Detection of UV and Flow rate was obtained at 240–254 nm and 1.0 mL min-1.  Prednisolone is diluted in methanol or buffer before use. The retention time for prednisolone was found to be 8.383 minutes. The linearity range, LOD, and LOQ were obtained at 0.1-2 mg L-1, 0.029 mg L-1, and 0.098 mg L-1, respectively. Additionally, the working range of prednisolone is between 0.1 and 100 mg L-1 (RSD < 5%). The method showed good precision and accuracy, making it suitable for the quantification of prednisolone. The technique utilizes high sensitivity and selectivity, meeting regulatory requirements, and achieves a recovery rate of more than 95%.

Graphical Abstract

Analysis and the determination of prednisolone in pharmaceutical products by a high-performance liquid chromatography method

Keywords

High-performance liquid chromatography
Analysis
Prednisolone
Pharmaceutical product
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Analytical Methods in Environmental Chemistry Journal
Volume 8, Issue 3
AMEC Publisher
Summer 2025
Pages 133-146